FastQCFastQC Report
Fri 24 Mar 2023
EGAF00001998392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001998392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79679577
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC4631010.5812041396755909No Hit
CGGCAGCACATATACTAAAATTGGAACAGATCGGAAGAGCACACGTCTGA1011880.1269936460631562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC985810.12372179134434913No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC951360.11939822421497043No Hit
TATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTAT932410.1170199485371264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)