FastQCFastQC Report
Wed 22 Mar 2023
EGAF00001998434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001998434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67394823
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC6136740.9105654895777381No Hit
GGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTT2882350.4276812181849636No Hit
GGCTGGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCC2766990.41056417642049453No Hit
GGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATC2670690.3962752450585114No Hit
TACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGA1523380.2260381335225111No Hit
TGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATAC1367600.20292359844909746No Hit
TATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTAT1114180.1653213036852994No Hit
ACCTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAG1096010.16262525090391586No Hit
CATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTAT1019060.1512074599557892No Hit
CGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATA973640.14446806989907815No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCT939900.1394617506451497No Hit
ACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCT910060.13503411085447914No Hit
AATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTAT886950.1316050640863023No Hit
TCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTA884320.13121482639697712No Hit
GGCTAGGCGGGTGTCCCCTTCCTCCCTCACCGCTCCATGTGCGTCCCTCC846430.12559273284240247No Hit
TTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGAT809370.12009379414795703No Hit
GGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACG769450.11417049051378916No Hit
CGGCAGCACATATACTAAAATTGGAACAGATCGGAAGAGCACACGTCTGA768460.1140235949577314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATCAGTT689950.10237433222430156No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)