FastQCFastQC Report
Tue 26 Sep 2023
EGAF00001999131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001999131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47111064
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTAT1359130.2884948639665621TruSeq Adapter, Index 7 (97% over 37bp)
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC768570.16314002163058766No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT639710.1357876357876358No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA586360.12446333201050182No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT522190.1108423278234599No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG515280.10937558107369427No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA508380.10791095696756074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)