FastQCFastQC Report
Thu 14 Sep 2023
EGAF00001999157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001999157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58715091
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCGAGAATCTCGTAT947230.1613264978163791TruSeq Adapter, Index 1 (97% over 36bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA924730.15749443358607756No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT782920.13334221009723038No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT722380.12303140260823235No Hit
CCCGTTACATCTTCCGCGCAGGCCGACTCGACTAGTGAGCTATTACGCTT588410.10021444061118802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)