FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001999710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001999710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94555
Sequences flagged as poor quality0
Sequence length245-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1811019.152873988683837No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1431615.140394479403522No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA57526.083231981386494No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA42874.533869176669663No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA33953.590502881920575No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA28102.9718153455660725No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA19912.1056527946697687No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA16891.7862619639363333No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG12541.3262122574163184No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA12511.3230395008196287No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG11801.2479509280313046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTA10841.1464227169372323No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG10681.1295013484215535No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA10241.082967585003437No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG7340.7762677806567606No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA7160.7572312410766221No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA6070.6419544180635609No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT5210.5510020622917878No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAC5000.5287927661149595No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGACTGTCCAGTA4170.44101316693987624No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA4100.43361006821426684No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3890.4114007720374385No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA3750.3965945745862197No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA3620.3828459626672307No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA3380.35746390989371263No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2780.29400877795991753No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2690.2844905081698482No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCATTTGCAGCTTAGCA2430.25699328433187035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCATTTACCGTCCAGTA1950.2062291787848342No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGGCCAGAT1910.20199883665591453No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA1870.19776849452699485No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGAA1780.1882502247369256No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1690.17873195494685631No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1630.1723864417534768No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1590.16815609962455713No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1530.1618105864311776No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC1500.15863782983448785No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGCTTAGCA1440.15229231664110834No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGTA1420.15017714557664852No Hit
CCTGTTTGATACACACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1380.14594680344772884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGAA1380.14594680344772884No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1230.13008302046428005No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1180.12479509280313046No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCCGGTCCAGCA1170.12373750727090052No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGGA1170.12373750727090052No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA1170.12373750727090052No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTCATGGCTTGGCA1160.12267992173867061No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA1120.11844957960975093No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA1110.11739199407752102No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGAA1080.11421923748083126No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1060.11210406641637143No Hit
CCTGTTTGCTCCACACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1060.11210406641637143No Hit
CCTGTTTGCTCCCCACGCTTTCGATCCTCAACGTCAGTTACCGTCCAGTA1020.10787372428745176No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA1010.10681613875522183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCCACGTCAGTTACCGTCCAGTA980.10364338215853207No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGCTGCACCCCAGAC960.10152821109407223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)