FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002000484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002000484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75122
Sequences flagged as poor quality0
Sequence length245-251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG920512.253401134155109No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA54667.276164106386944No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG49046.52804770905993No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG39675.280743324192646No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG36694.88405527009398No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA33134.41015947392242No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG28923.8497377599105453No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA23453.1215888820851414No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG15512.0646415164665477No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG14451.9235377119884989No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG10411.3857458534117835No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC8761.1661031388940657No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG7521.0010383110140837No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTCGAGGCCCAGAA6020.8013631159979766No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT5520.7348047176592742No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG4710.6269801123505764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG4400.585713905380581No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA4350.5790580655467107No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4270.5684087218125183No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG3740.4978568195734938No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG3500.46590878837091665No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG3460.4605841165038205No Hit
CCTATTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATGGCCCAGCA3160.420649077500599No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCTCTGTCTAGGT3110.41399323766672874No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2970.39535688613189207No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2850.37938287053060354No Hit
CCTGTTCGATACCCGCGCTTTCGAGCATGAGCGTCAGTAGCGCTCCCGTC2430.3234738159260936No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTA2420.3221426479593195No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2330.31016213625835304No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2140.2848699448896462No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTACAGACCAGAC2000.2662335933548095No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1920.2555842496206171No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1880.2502595777535209No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACTAGAC1600.2129868746838476No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1590.21165570671707357No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGGCCAGAT1550.2063310348499774No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1550.2063310348499774No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1430.1903570192486888No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1360.18103884348127047No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1330.1770453395809483No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1280.1703894997470781No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1260.16772716381353No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1240.1650648278799819No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1170.15574665211256356No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC1130.15042198024546738No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG1130.15042198024546738No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1120.1490908122786933No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCTCAGAG1100.14642847634514525No Hit
CCTATTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1030.13711030057772688No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCTCAGCA980.13045446074385667No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACGGCCCAGAG950.1264609568435345No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG950.1264609568435345No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACCTCAGCGTCAGTGTTGGCCCAGAA940.12512978887676046No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGTCCAGA930.12379862090998643No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA880.11714278107611618No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCTCAGAG850.11314927717579404No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCATACCAGA830.11048694124224595No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA820.1091557732754719No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC780.1038311014083757No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGTTCTCGGCCCAGAG770.10249993344160166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)