FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002000524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002000524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257
Sequences flagged as poor quality0
Sequence length246-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA554.375497215592681No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA262.0684168655529036No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA241.9093078758949882No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA201.591089896579157No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG161.2728719172633254No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA110.8750994431185362No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG100.7955449482895784No Hit
CCTTACCGCGGCTGCTGGCACACAATTACCATACAGATAGTGTGTAGATC100.7955449482895784No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA90.7159904534606205No Hit
CCTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAGATC80.6364359586316627No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA80.6364359586316627No Hit
CCTGTTTGCTCCCTTACCGCGGCGGCTGGCACACAATTACCATACAGATA70.5568814638027049No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA70.5568814638027049No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG60.47732696897374705No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTAACAGTCCAGTG50.3977724741447892No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG50.3977724741447892No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA50.3977724741447892No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA50.3977724741447892No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA50.3977724741447892No Hit
CCTGTTTGCTCCCTTACCGCGGCTGCTGGCACACAATTACCATACAGATA50.3977724741447892No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA50.3977724741447892No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA50.3977724741447892No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG40.31821797931583135No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA40.31821797931583135No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA40.31821797931583135No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG30.23866348448687352No Hit
CCTGTTTGCTACCCACGCTTTCGTTCCTCAGCGTCAGATTTGTGTCCAGT30.23866348448687352No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA30.23866348448687352No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTGCGGCA30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCATTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA30.23866348448687352No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA30.23866348448687352No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA30.23866348448687352No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA30.23866348448687352No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGCA30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA30.23866348448687352No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGTA30.23866348448687352No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG30.23866348448687352No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG30.23866348448687352No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA20.15910898965791567No Hit
CCTTCTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAG20.15910898965791567No Hit
CCTTTACCGCGGCTGCTGGCACACAATTACCATACAGATAGTGTGTAGAT20.15910898965791567No Hit
CCTCTTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAG20.15910898965791567No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA20.15910898965791567No Hit
CCTACTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAG20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGCA20.15910898965791567No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA20.15910898965791567No Hit
CCTGTTCGCTTCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA20.15910898965791567No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA20.15910898965791567No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA20.15910898965791567No Hit
CCTCGTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAG20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACCGTCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGCCAGTCGCATCCCAGGG20.15910898965791567No Hit
CCTAAAGCTCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT20.15910898965791567TruSeq Adapter, Index 5 (100% over 38bp)
CCTGTTCGACACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA20.15910898965791567No Hit
CCTGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20.15910898965791567No Hit
CCTTACCGCGGCTGCTGGCACACAATTACCATACAGATAGTGTTTAGATC20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT20.15910898965791567No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTTATCGTCCAGTA20.15910898965791567No Hit
CCTGTTCGCTACCCACGCTTTCGTCCATCAGCGTCAATCCATTAGTAGTA20.15910898965791567No Hit
CCTTAAGTTCGAGATCGGAAGAGCACACGTCTGAATTACCGCGGCTGCTG20.15910898965791567Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGGAAAGCCCCAGCC20.15910898965791567No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG20.15910898965791567No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA20.15910898965791567No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA20.15910898965791567No Hit
CCTTTACCGCGGCGGCTGGCACACAATTACCATACAGATAGTGTGTAGAT20.15910898965791567No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA20.15910898965791567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)