FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002000588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002000588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72920
Sequences flagged as poor quality0
Sequence length245-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA1597921.91305540318157No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1153115.81321996708722No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA801410.990126165661No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG32004.3883708173340645No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG27883.823368074602304No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATAGCCCAGAG20702.8387273724629734No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG19882.726275370268788No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG16062.202413603949534No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA10531.4440482720789907No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG4250.5828304991771804No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGTG3770.5170049369171695No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2560.3510696653867252No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG2290.31404278661546897No Hit
CCTGTTTTCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA2080.28524410312671417No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGGTCCAGAA1780.24410312671420734No Hit
CCTGTTTTCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1780.24410312671420734No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTCTTCGTCCAGGG1660.22764673614920464No Hit
TCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA1610.22078990674712015No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTATTGGTCCAGAA1400.19199122325836532No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATGGTCCAGAA1280.1755348326933626No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGTA1270.1741634668129457No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGCA1220.16730663741086121No Hit
TCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1100.1508502468458585No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1100.1508502468458585No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1060.1453647833241909No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGT1050.143993417443774No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1050.143993417443774No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCTAGAA990.13576522216127263No Hit
TCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA960.13165112452002192No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA950.13027975863960506No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA940.12890839275918814No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA880.12068019747668679No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG880.12068019747668679No Hit
CCCGTTCGCTCCCCTTGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA870.11930883159626988No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGTG830.11382336807460232No Hit
CCTGTTTGCTTCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGTCCAGAA830.11382336807460232No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGCG810.11108063631376851No Hit
CCCGTTCTCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA770.10559517279210093No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC760.10422380691168404No Hit
CCTGTTTGCTCCCCACGCTCTCGTGCCTCAGCGTCAGTAATGGTCCAGAA730.10010970927043335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)