FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002000954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002000954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95063
Sequences flagged as poor quality0
Sequence length243-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA79278.338680664401501No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA46994.943037774949245No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA42794.501225503087427No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA30363.19367156517257No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA30283.185256093327583No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA28302.9769731651641544No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA27382.8801952389468037No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA23132.433123297181869No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA21992.313202823390804No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG20232.12806244280109No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA19722.0744138097892977No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA18671.963960741823843No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA16761.7630413515247783No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA16111.6946656427842588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA13931.4653440350083629No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA11421.2013086058718954No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA9951.0466743107202592No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA8350.878364873820519No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8200.8625858641111683No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA7780.8184046369249866No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC7710.8110410990606229No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT7140.7510808621650905No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA6550.6890167573083114No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA6410.674289681579584No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6170.649043266044623No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT6070.6385239262383893No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5970.6280045864321555No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5940.6248487844902854No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5920.6227449165290386No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA5830.6132775107034283No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA5510.5796156233234803No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA4800.5049283106992205No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA4750.4996686407961037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA4030.4239293941912205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA3950.4155139223462336No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC3920.41235812040436337No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA3660.38500783690815565No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA3280.34503434564446733No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA3240.3408266097219738No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3190.335566939818857No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA3180.33451500583823357No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA3040.31978793010950635No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2870.3019050524389089No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2840.2987492504970388No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2770.29138571263267515No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA2730.28717797671018164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2570.2703470330202076No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA2450.25772382525272713No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA2450.25772382525272713No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA2040.21459453204716872No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2000.2103867961246752No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG1640.17251717282223367No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1570.16515363495787005No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT1560.16410170097724666No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTCACCGTCCAGTA1550.1630497669966233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA1370.14411495534540253No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT1320.13885528544228565No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTACAGGTTAGTA1310.13780335146166225No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1270.13359561553916877No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA1230.12938787961667525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA1140.11992047379106488No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1130.1188685398104415No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1120.11781660582981812No Hit
CCCATTTGCTACCCCGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA1100.11571273786857136No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1080.1136088699073246No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1080.1136088699073246No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1050.1104530679654545No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1020.10729726602358436No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA1020.10729726602358436No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTAACAGTCCAGTG1010.10624533204296098No Hit
CCTATTTGCTCCCCACGCTTTCGAGAATGAGCGTCAGTTGCGGCCCAGAC1000.1051933980623376No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA970.10203759612046749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)