FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002001118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002001118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489
Sequences flagged as poor quality0
Sequence length246-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA5229.509928948806705No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5139.345964656585899No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3446.267079613773001No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA2143.8987065039169244No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1663.0242302787392967No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1622.951357259974494No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1162.1133175441792678No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1091.9857897613408635No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1021.8582619785024597No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA971.7671707050464565No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG881.6032064128256511No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA741.348150847148843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA721.311714337766442No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA701.275277828384041No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG671.2206230643104392No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG581.0566587720896339No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA571.0384405173984332No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA540.9837857533248315No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA520.9473492439424303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA450.8198214611040262No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA430.7833849517216251No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG410.7469484423392239No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA400.7287301876480233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA380.6922936782656222No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG380.6922936782656222No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG370.6740754235744215No Hit
CCTGTTACGCTAATTACCGCGGCGGCTGGCACACAATTACCATACACTCA350.6376389141920205No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA350.6376389141920205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA310.5647658954272181No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA310.5647658954272181No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG250.45545636728001454No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA250.45545636728001454No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA240.437238112588814No Hit
CCTGTTACGCTAATTACCGCGGCTGCTGGCACACAATTACCATACACTCA240.437238112588814No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG230.4190198578976134No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA200.36436509382401167No Hit
CCTGTTACGCTTACCGCGGCTGCTGGCACACAATTACCATACACTCACGG200.36436509382401167No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT190.3461468391328111No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA180.3279285844416105No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA180.3279285844416105No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT160.2914920750592093No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA160.2914920750592093No Hit
CCTGTTACGCTAACGCAACTTACCGCGGCGGCTGGCACACAATTACCATA160.2914920750592093No Hit
CCTGTTACCGCGGCGGCTGGCACACAATTACCATACACTCACGGTGTAGA150.27327382036800874No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG150.27327382036800874No Hit
CCTGTTACGCTAACGTTACCGCGGCTGCTGGCACACAATTACCATACACT140.25505556567680815No Hit
CCTGTTACGCTAACGCAACTGAGTTTACCGCGGCTGCTGGCACACAATTA140.25505556567680815No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT140.25505556567680815No Hit
CCTGTTACGCTAACGCAACTGAGTTTACCGCGGCGGCTGGCACACAATTA140.25505556567680815No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA140.25505556567680815No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA130.23683731098560756No Hit
CCTGTTACGCTTACCGCGGCGGCTGGCACACAATTACCATACACTCACGG120.218619056294407No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA110.2004008016032064No Hit
CCTGTTACGTTACCGCGGCGGCTGGCACACAATTACCATACACTCACGGT110.2004008016032064No Hit
CCTGTTACGCTAACGCAACTTACCGCGGCTGCTGGCACACAATTACCATA110.2004008016032064No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA110.2004008016032064No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA110.2004008016032064No Hit
CCTGTTACGCTAACGTTACCGCGGCGGCTGGCACACAATTACCATACACT100.18218254691200583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG100.18218254691200583No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTCAAGTCCAGAA100.18218254691200583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGGCCAGAG90.16396429222080525No Hit
CCTGTTACGCTAACGCAACTGAGTTACCGCGGCTGCTGGCACACAATTAC90.16396429222080525No Hit
CCTGTTACGCTAACGCAACTGAGTCGGAGACACGTTACCGCGGCTGCTGG90.16396429222080525No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA90.16396429222080525No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTGCCGCTCCCGTC90.16396429222080525No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA90.16396429222080525No Hit
CCTGTTACGTTACCGCGGCTGCTGGCACACAATTACCATACACTCACGGT90.16396429222080525No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA90.16396429222080525No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA80.14574603752960466No Hit
CCTTACCGCGGCTGCTGGCACACAATTACCATACACTCACGGTGTAGATC80.14574603752960466No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT80.14574603752960466No Hit
CCTGTTACGCTAACGCAACTGAGTTACCGCGGCGGCTGGCACACAATTAC80.14574603752960466No Hit
CCTGTTACCGCGGCTGCTGGCACACAATTACCATACACTCACGGTGTAGA70.12752778283840407No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG70.12752778283840407No Hit
CTTACCGCGGCTGCTGGCACACAATTACCATACACTCACGGTGTAGATCT70.12752778283840407No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA70.12752778283840407No Hit
CCTGTTACGCTAACGCAATTACCGCGGCTGCTGGCACACAATTACCATAC70.12752778283840407No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG70.12752778283840407No Hit
CCTTACCGCGGCGGCTGGCACACAATTACCATACACTCACGGTGTAGATC60.1093095281472035No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT60.1093095281472035No Hit
CCTGTTCGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTGTGGCCCAGAA60.1093095281472035No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG60.1093095281472035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGTCTCAGCGTCAGTTATCGTCCAGTA60.1093095281472035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)