FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002001555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002001555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92735
Sequences flagged as poor quality0
Sequence length244-251
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA2962531.945867256160028No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2080322.432738448266566No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA70067.554860624359734No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40584.375909850649701No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA39014.206610233460937No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA34653.736453334771122No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA21532.321669272658651No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCATCTA11601.2508761524774896No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAACGTA8140.8777699897557556No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5860.6319081253032836No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA5060.545640804442767No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA5050.5445624629320106No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA4770.5143689006308297No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA2480.2674286946676012No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA2390.2577236210707931No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCGCGCA2270.24478352294171565No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA2220.23939181538793333No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA2030.2189033266835607No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1650.17792634927481535No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1630.1757696662533024No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAGGCGCGTCTA1580.17037795869952016No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA1420.15312449452741683No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1390.14988946999514746No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTACGCA1350.14557610395212164No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCACGCA1300.14018439639833935No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCCTA1300.14018439639833935No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1260.13587103035531353No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1190.12832263978001834No Hit
TACGTATGTCGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1140.12293093222623605No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGCGTCTA1080.11646088316169731No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA1070.11538254165094086No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTCTA1010.10891249258640212No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA980.10567746805413274No Hit
TACGGATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA970.10459912654337629No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTGTA960.10352078503261984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)