FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002001556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002001556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92735
Sequences flagged as poor quality0
Sequence length243-251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA2672528.818676874966304No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1392015.010513829729877No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG55786.014988946999514No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG34653.736453334771122No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA32713.5272550816843697No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA19052.0542405779910498No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG18782.0251253572006256No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA13241.4277241602415485No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG7880.8497331104760877No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCCAGTG7270.7839542783199439No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA6100.6577883215614385No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTTAGCGTCAGTATCTGTCCAGTA5900.6362214913463093No Hit
CCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTT4390.47339192322208445No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA4170.44966840998544244No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG3710.4000647004906454No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA3620.3903596268938373No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3280.35369601552811775No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG2760.29762225696878203No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2690.2900738663934868No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG2550.27497708524289644No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTATCTGTCCAGTA2440.2631153286245754No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCTAGTA2360.2544885965385238No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG2360.2544885965385238No Hit
CCTGTTTGCTACCCACACTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA2200.23723513236642044No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2140.23076508330188172No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGCGTCAGTTATGCTACAGTA2040.21998166819431714No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCATTATCTGTCCAGTA1900.20488488704372676No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1790.19302313042540573No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1660.1790046907855718No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTACATTCCCAAGG1640.17684800776405887No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1510.16282956812422494No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTTCAGTCCAGTG1420.15312449452741683No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTCTAGCGTCAGTATCTGTCCAGTA1350.14557610395212164No Hit
CCTGTTTGATACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1290.1391060548875829No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCATTTGCAGTCCAGTG1260.13587103035531353No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1220.1315576643122877No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1180.1272442982692619No Hit
ACTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1160.12508761524774895No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1160.12508761524774895No Hit
CCTGTTTGCTACCCACGCTCTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1120.12077424920472314No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1090.11753922467245377No Hit
CCTATTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1030.11106917560791503No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGTCCAGTG1010.10891249258640212No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG990.1067558095648892No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA980.10567746805413274No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG950.10244244352186338No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTCATGTCCCAGAG940.1013641020111069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)