FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002002474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002002474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92768
Sequences flagged as poor quality0
Sequence length244-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1239913.36560020696792No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA75508.13858226974819No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA57226.1680751983442565No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG24442.6345291479820627No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA18622.0071576405657123No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA16411.7689289410141429No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA15121.6298723697826836No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA13531.4584770610555364No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA12401.3366678164884442No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT11121.1986892031735081No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA9641.0391514315281132No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA9521.0262159365298378No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9491.022982062780269No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG7520.8106243532252502No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA7490.8073904794756812No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA7040.758882373232149No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA6160.6640220765781304No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC6020.6489306657468092No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA5740.6187478440841669No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTATTTGCCCAGGA5470.5896429803380476No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG4980.5368230424284236No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA4960.5346671265953777No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG4850.5228095895136253No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4630.4990945153501207No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG4610.49693859951707486No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG4600.4958606416005519No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA4500.4850810624353225No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4470.48184718868575377No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA4450.47969127285270785No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTGGCA3900.4204035874439462No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA3780.4074680924456709No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3510.3783632286995516No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA3410.36758364953432215No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3340.36003794411866163No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA3290.3546481545360469No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA3150.33955674370472577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2960.3190755432907899No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2950.317997585374267No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA2770.2985943428768541No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2680.28889272162814766No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA2290.246852362883753No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA2270.24469644705070714No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2260.2436184891341842No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAA2150.23176095205243186No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG2020.21774749913763364No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA1970.21235770955501898No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG1970.21235770955501898No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCTCTGTCTAGGT1920.2069679199724043No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTTGCA1860.20050017247326665No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG1750.18864263539151432No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA1720.1854087616419455No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1700.1832528458088996No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1660.17894101414280786No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA1650.17786305622628493No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA1550.16708347706105553No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGGTCGTCCAGTA1520.1638496033114867No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC1520.1638496033114867No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1490.1606157295619179No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1480.15953777164539495No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG1440.1552259399793032No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1430.15414798206278027No Hit
CCTATTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAACAGCCCAGCA1370.14768023456364263No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1360.1466022766471197No Hit
CCTGTTTGCTACCCACGCTTTCGCATATCAGCGTCAGTATTTGCCCAGGA1260.1358226974818903No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG1250.13474473956536737No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA1240.13366678164884443No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1210.1304329078992756No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1210.1304329078992756No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1200.12935494998275268No Hit
CCTATTTGCTCCCCACGCTTTCGTCCCTGACCGTCAGTGATGGCCCAGCA1180.1271990341497068No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1170.12612107623318386No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGTA1170.12612107623318386No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1150.12396516040013797No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCTCAGGA1090.11749741290100035No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTA1020.10995170748533978No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1010.10887374956881682No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA990.10671783373577094No Hit
CCTATTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTACAGCCCAGCA980.10563987581924802No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTCGAGGCCCAGAA980.10563987581924802No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG970.10456191790272507No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA960.10348395998620215No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA930.10025008623663333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)