FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002002686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002002686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86452
Sequences flagged as poor quality0
Sequence length242-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA69658.056493776893536No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA46505.378707259519733No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG39314.547031878961736No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA38694.47531578216814No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG32333.7396474344144726No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA27543.1855827511220096No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA26503.065284782306945No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA25882.9935686855133485No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA23282.692823763475686No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA18772.171146994864202No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA13831.5997316429926431No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA11861.3718595289871838No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA11781.3626058390783324No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA10751.243464581501874No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG10641.2307407578772034No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG10001.1567112386063942No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA9971.153241104890575No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA8721.0086522000647757No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA7770.8987646323971683No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA6990.8085411557858696No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT6430.7437653264239116No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA6230.7206311016517837No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTCTGGCG6170.7136908342201453No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC5520.6385046037107297No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA5320.6153703789386017No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5120.5922361541664739No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA5050.5841391754962291No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA4980.5760421968259843No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA4760.5505945495766437No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA4600.5320871697589413No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4110.475408319067228No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3460.40022208855781244No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA3420.3955952436033868No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG3240.37477444130847176No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA2790.322722435571184No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2760.31925230185536485No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA2730.3157821681395456No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGACCGTCCAGTA2640.3053717669920881No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA2630.3042150557534817No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2550.29496136584463056No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2330.2695137185952899No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA2230.25794660620922594No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCA1850.21399157914218295No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA1720.19895433304029983No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1600.1850737981770231No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTACAGTCCAGAA1590.18391708693841669No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA1590.18391708693841669No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1420.16425299588210798No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1370.15846943968907604No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTA1350.15615601721186323No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1290.14921574978022487No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1240.1434321935871929No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1220.14111877110998008No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA1220.14111877110998008No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1220.14111877110998008No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1190.13764863739416092No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGTA1190.13764863739416092No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAGTGTCCAGCA1140.13186508120112897No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1130.13070836996252255No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1090.12608152500809697No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1060.1226113912922778No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCATA1050.12145468005367141No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1050.12145468005367141No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACCGTCCAGTA1050.12145468005367141No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1040.120297968815065No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1030.11914125757645862No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCATACCAGA1020.11798454633785221No Hit
CCTGTTTGCTCCCCACACTTTCGCTCCTCAGCGTCAGTTAATGTCCAGCA1020.11798454633785221No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA990.11451441262203303No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA950.10988756766760746No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA940.10873085642900107No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA930.10757414519039467No Hit
CCTATTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTTTGGCCCAGCA890.1029473002359691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)