FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002003058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002003058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44721
Sequences flagged as poor quality0
Sequence length240-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA589313.177254533664273No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA42029.396033183515575No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA28186.301290221596117No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20894.671183560296058No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA11862.6519979428009215No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG10622.374723284363051No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA9452.1131012276111893No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG7801.7441470450124101No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA7791.7419109590572663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG7011.567496254556025No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA6721.5026497618568457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA5531.236555533194696No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA4981.1135708056617697No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA4370.9771695623979787No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4140.9257395854296638No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA4100.9167952416090875No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC3790.8474765769996199No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3720.8318239753136111No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3610.8072270298070258No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA3460.773685740479864No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3320.7423805371078465No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3130.6998949039601082No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3090.6909505601395318No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2900.6484649269917936No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA2760.6171597236197759No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA2710.6059792938440554No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2610.5836184342926142No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2600.58138234833747No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2590.5791462623823259No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2590.5791462623823259No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGCGTCAATTACGGCCCAGTA2470.5523132309205966No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2200.49193891013170543No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1890.42262024552223787No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA1800.4024954719259408No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA1790.4002593859707967No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1650.3689541825987791No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1600.3577737528230585No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1520.3398850651819056No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1400.3130520337201762No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA1260.28174683034815856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1250.2795107443930145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1130.2526777129312851No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1110.24820554102099684No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1090.24373336911070864No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1080.2414972831555645No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA1050.23478902529013215No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1050.23478902529013215No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1030.2303168533798439No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1020.22808076742469982No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGATAGCCAGAT1000.2236085955144116No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAACGCCCAGCA990.22137250955926746No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA990.22137250955926746No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA970.2169003376489792No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT910.2034838219181145No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG910.2034838219181145No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA890.1990116500078263No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA850.19006730618724982No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGCCCAGTA810.18112296236667338No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA790.17665079045638515No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA750.16770644663580866No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCTAGCA740.16547036068066456No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA690.15428993090494397No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT680.15205384494979987No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG620.13863732921893518No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA620.13863732921893518No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA610.13640124326379108No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA610.13640124326379108No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT600.13416515730864695No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA560.1252208134880705No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA550.12298472753292636No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA510.11404038371234991No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA490.10956821180206167No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA480.10733212584691756No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA470.10509603989177345No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG460.10285995393662932No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA460.10285995393662932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)