FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002003120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002003120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83385
Sequences flagged as poor quality0
Sequence length245-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA844210.124123043712897No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG38594.6279306829765545No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA28943.4706481981171677No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA28683.439467530131319No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA26603.190022186244528No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA23292.7930682976554535No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21272.5508184925346287No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG19622.3529411764705883No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG17962.1538646039455536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA14531.7425196378245487No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC10311.2364334112850033No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA9641.156083228398393No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA9511.1404928944054686No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA9371.12370330395155No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGCGTCAGTTGCAGACCAGGC9061.0865263536607304No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA8881.064939737362835No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA7710.9246267314265156No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA7560.9066378845116028No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA7540.9042393715896144No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA7450.8934460634406668No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA7280.8730587036037657No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA6870.8238891887030041No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA5760.6907717215326498No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA5600.6715836181567428No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA4680.5612520237452779No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA4160.49889068777358037No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA4080.4892966360856269No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT3670.4401271211848654No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA3450.4137434790429933No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3150.37776578521316784No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA3140.37656652875217367No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTAATGCCCCGGTA3070.3681717335252144No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG3070.3681717335252144No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2730.3273970138514121No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA2490.29861485878755173No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA2480.2974156023265575No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTGACACTCCCGTG2460.29501708940456917No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA2330.27942675541164474No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG2130.25544162619176114No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2030.24344906158181925No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1990.23865203573784255No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1980.23745277927684835No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1960.23505426635486No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGTTCTCGGCCCAGAG1720.20627211129099957No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1700.20387359836901123No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1670.20027582898602866No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCCAGTG1640.1966780596030461No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA1570.18828326437608683No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG1560.18708400791509264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA1480.17748995622713917No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA1470.17629069976614498No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1370.16429813515620317No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1360.16309887869520898No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGGG1360.16309887869520898No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCTCAGAG1340.1607003657732206No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCATTTACCGTCCAGTA1310.15710259639023805No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1310.15710259639023805No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1270.15230557054626132No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1260.15110631408526712No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1190.14271151885830785No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1120.13431672363134856No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1050.1259219284043893No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1020.12232415902140673No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA980.11752713317743No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA980.11752713317743No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATAGTTTAGTA900.10793308148947653No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG890.10673382502848233No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA870.10433531210649397No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA860.10313605564549978No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA860.10313605564549978No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA850.10193679918450561No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG850.10193679918450561No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGCA840.10073754272351143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)