FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002003143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002003143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80122
Sequences flagged as poor quality0
Sequence length248-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA3930049.05019844736776No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA982212.258805321884127No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA808110.085869049699209No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA71278.89518484311425No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA16702.0843214098499785No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10681.3329672249819025No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA9121.1382641471755572No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4990.6228002296497841No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGCA2170.2708369736152368No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1970.24587504056314122No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAGGCGCACGCA1890.235890267342303No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1830.2284016874266743No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1720.21467262424802175No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA1300.1622525648386211No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1300.1622525648386211No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1260.15726017822820199No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCG1120.1397868250917351No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA1070.13354634182871122No Hit
TACGGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGCACGCA1050.13105014852350166No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGTGCGTAAAGCGCACGCA970.12106537530266344No Hit
TACGGAGGGTGCAAGCGTTAATCGGAGTTACTGGGCGTAAAGCGCACGCA950.11856918199745388No Hit
TACGGAGGGTGCAAGCGTTAATCGGGATTACTGGGCGTAAAGCGCACGCA890.1110806020818252No Hit
TACGGAGGGTGCAGGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA880.10983250542922045No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCACGCA830.10359202216619655No Hit
TACGGAGGGTGCAAGCGCTAATCGGAATTACTGGGCGTAAAGCGCACGCA820.10234392551359177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)