FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002003530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002003530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66211
Sequences flagged as poor quality0
Sequence length246-251
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA62029.367023606349397No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA44686.748123423600308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA43246.530636903233601No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA40116.057905786047636No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA28934.369364607089456No Hit
CCCGTTCGCTACCCTAGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA20323.0689764540635243No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT18582.8061802419537543No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17862.6974369817704007No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG15602.3561039706393196No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA15332.3153252480705624No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG13992.1129419582848774No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA13552.0464877437283833No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG11531.7414024859917536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA11451.7293199015269367No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG8521.286795245503013No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG8461.2777333071544004No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA7911.1946655389587832No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG6620.9998338644636088No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA6220.9394209421395237No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA6130.9258280346166045No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA5570.8412499433628854No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA5400.8155744513751493No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5010.7566718521091661No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4820.7279757140052258No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4070.6147014846475661No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA4010.6056395462989533No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3440.519551131987132No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA3350.5059582244642129No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA3270.4938756399993959No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA3220.48632402470888525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA3110.4697104710697618No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2780.4198698101523916No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG2610.39419431816465544No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2340.35341559559589797No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2170.3277401036081618No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA2160.3262297805500597No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA2040.30810590385283415No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1980.29904396550422135No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1870.28243041186509793No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1810.2733684735164852No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1760.26581685822597456No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG1630.24618265847064688No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1430.21597619730860432No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1420.21446587425050218No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCTAGAA1330.20087296672758304No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1330.20087296672758304No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA1290.19483167449517452No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1230.18576973614656175No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1090.16462521333313196No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA970.14650133663590642No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA950.14348069051970216No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA940.14197036746160005No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA920.1389497213453958No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA880.13290842911298728No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA850.12837745993868088No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA800.12082584464817023No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA760.11478455241576173No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT740.11176390629955749No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA710.10723293712525109No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCTAGCA690.10421229100904685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)