FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002012979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002012979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424365
Sequences flagged as poor quality0
Sequence length35-301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG162440.6700311215514165Illumina Single End PCR Primer 1 (100% over 50bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32230.13294202811870323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)