FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102223
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG2885928.231415630533245No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG1722816.853350028858475No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG1171811.463173649765707No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG84108.227111315457382No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG50014.892245385089461No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG17991.7598779139723937No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG12491.2218385294894496No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG12331.2061864746681277No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAATACATG10401.0173835633859307No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTAACACATG9040.8843410974046937No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACATG6500.6358647271162067No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGATAGGCTTAACACATG5090.4979309940033065No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTAGCGGCATGCCTTACACATG4520.44217054870234684No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG4090.40010565137004395No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG3950.38641010340138715No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG3710.36293202116940415No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG3660.35804075403774105No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG3130.30619332244211184No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGAAATGTGGATACCGGCAT2910.2846717470627941No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG2560.2504328771411522No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG2370.23184606204083227No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAACACATG2080.20347671267718614No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGTGGCATGCCTAACACATG1930.18880291128219678No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG1840.17999863044520312No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGGATAAGGCATG1690.16532482905021376No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG1600.15652054821322012No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAACACATG1510.14771626737622648No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG1460.14282500024456335No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1410.13793373311290022No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAACACATG1360.1330424659812371No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACACG1340.13108595912857185No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1310.12815119884957396No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGAGTGCCTAACACATG1220.11934691801258034No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAACACATG1160.11347739745458459No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAACACATG1160.11347739745458459No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAACACATG1130.11054263717558671No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACGCATG1100.10760787689658882No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACGTG1080.10565137004392357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)