FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196127
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG13921370.98104799441178No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG127496.500379855909691No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG49142.505519382848868No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG14570.7428859871409852No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG12640.6444803622142795No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG12100.6169471821829733No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG11490.5858448862216828No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG10500.5353673894976214No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG6470.3298882866713915No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG5440.27737129513019626No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGTAGGCCTAACACATG4830.24626899916890588No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG4460.22740367211041826No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG4360.22230493506758378No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG4160.2121074609819148No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG3860.1968112498534113No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG3770.1922223865148603No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG3500.17845579649920715No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG3410.1738669331606561No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG2990.15245223758075124No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAATACATG2980.1519423638764678No Hit
AGAGTTCGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2920.14888312165076711No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAATACATG2780.14174488979079883No Hit
GAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATGC2700.1376659001565312No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG2670.13613627904368086No Hit
AGAGTCTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2580.13154741570512984No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAGTACATG2550.1300177945922795No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTCATACATG2530.1289980471837126No Hit
AGAGTTTGATCCCGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2520.12848817347942915No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCATGCCTAATACATG2460.12542893125372845No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCTTAATACATG2420.12338943643659467No Hit
AGAGCTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2370.12084006791517742No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAATACATG2340.11931044680232707No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATGCATG2340.11931044680232707No Hit
AGAGTTTGATCCTGGCCCAGAACGAACGCTGGCGGCATGCCTAATACATG2320.11829069939376016No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2220.11319196235092567No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATCCATG2160.11013272012522497No Hit
AGAGTTTGACCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2130.10860309901237464No Hit
AGAGTTTGATCCTGGCTCAGAACGCACGCTGGCGGCATGCCTAATACATG2050.10452410937810705No Hit
AGGGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2000.10197474085668981No Hit
GGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1980.10095499344812289No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)