FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171171
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC6995340.86731981468824No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2543814.861162229583282No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT62873.6729352518826204No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC37542.1931285089179826No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC32711.9109545425334897No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC29751.7380280538175277No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC21561.2595591542959963No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC18771.0965642544589913No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC14200.8295797769481981No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC12870.7518796992481203No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTGGGTACC7590.4434162328899171No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC7120.4159583106951528No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC6380.3727266885161622No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC5570.3254055885634833No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC5420.3166424219055798No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC5220.3049581996950418No Hit
TTACCGCGGCTGCTGGCACTCGCAATGGGGGTGTTAGCCGCCTGGCTGAT4860.28392659971607337No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC4840.2827581774950196No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4360.25471604418972843No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC4230.2471212997528787No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3650.21323705534231852No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTGTCGGTAAC3500.20447388868441502No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC3420.1998001998001998No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC3210.1875317664791349No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC3070.17935281093175828No Hit
TACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAACG2730.1594896331738437No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTAAC2690.1571527887317361No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATCCTGTCGGTAAC2670.1559843665106823No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCCTATTCTGTCGGTAAC2640.1542317331791016No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGCGCTTATTCTGTCGGTAAC2630.1536475220685747No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC2560.1495580442948864No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC2430.14196329985803668No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC2400.140210666526456No Hit
TTACCGCGGCTGCTGGCACTCGCAATGGGGGTGTTGGCCGCCTGGCTGAT2330.13612118875276769No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA2290.13378434431066008No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCGTCGGTAAC2260.1320317109790794No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTACC2010.11742643321590691No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC1980.11567379988432619No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1790.10457378878431509No Hit
TTACCGCGGCTGCTGGCACAGAGCTAGCCGGTGCTTATTCTGTCGGTAAC1790.10457378878431509No Hit
TTACCGCGGCTGCTGGCATAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1770.10340536656326131No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAGC1770.10340536656326131No Hit
TTACCGCGGCTGCTGGCCCAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1750.10223694434220751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)