FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168860
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG10296760.977733033282014No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG146668.685301433139879No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG99865.913774724623949No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG49632.939121165462513No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG19731.1684235461328911No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG16300.965296695487386No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG12760.7556555726637451No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG5210.30853961861897433No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG4770.28248252990643136No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG4600.2724150183584034No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG4390.25997868056378065No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG4170.2469501362075092No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG4060.24043586402937345No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGTGCCTAATACATG3910.23155276560464289No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG3580.2120099490702357No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG3400.20135023096055907No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG3340.19779699159066683No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG3090.18299182754944926No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG3030.17943858817955702No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG3020.17884638161790833No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG3000.17766196849461094No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG2910.1723321094397726No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG2540.15042046665877057No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAGCACATG2460.14568281416558096No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2430.14390619448063485No Hit
AGAGTTTGATCCTGGCTCAGGTCGCCTTTGGCTATGCGTGCAGCCACGGC2340.1385763354257965No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCCAACACATG2290.135615302617553No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGCGTACATAAGACATG2230.1320620632476608No Hit
GAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGC2060.12199455169963284No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1920.11370365983655098No Hit
AGAGTTTGACCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1830.10837380078171266No Hit
AGAGTTTGATCCTGGCCCAGATTGAACGCTGGCGGCAGGCCTAACACATG1830.10837380078171266No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTCACACATG1830.10837380078171266No Hit
AGAGCTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1740.10304394172687434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)