FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167278
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3340919.972142182474684No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC130937.827090233025263No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC108386.479034899986848No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC59983.5856478437092743No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT44092.635732134530542No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC39142.3398175492294264No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC34812.0809670129963296No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC31141.8615717548033812No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC29621.7707050538624327No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC21681.2960461028945827No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC19471.1639307021843877No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC17691.0575210129245927No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC13120.7843231028587142No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGAGGCTGATTCGATAGGTACC12800.7651932710816725No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC12610.7538349334640538No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT11160.6671528832243331No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC10050.6007962792477194No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC8320.4973756262030871No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC7400.4423773598440919No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGGAGCTTTCTTCTATGGTACT7100.4244431425531152No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC6900.41248699769246405No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC6710.4011286600748455No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTTTCTTATGGTACC6450.385585671755999No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGAGGCTTATTCCTCTGGTACC5630.3365654778273294No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATGAGTTACC5450.3258049474527433No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACAGGTACC5410.3234137184806131No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC5130.30667511567570155No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGCTACC5080.3036860794605387No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCAGGTACC5030.300697043245376No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC4880.2917299345998876No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGCTCCTACC4720.28216501871136673No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC4660.27857817525317136No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC4660.27857817525317136No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTATTACTCAGGTACCG4580.2737957173089109No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC4560.2726001028228458No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCGAGTACC4380.2618395724482598No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGTCCTTATTCGTATGGTACT4340.25944834347612955No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCCGGTACC4250.25406807828883654No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC4220.2522746565597389No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC4010.2397207044560552No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTATTACTCTGGTACCG4000.23912289721302263No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC4000.23912289721302263No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCTAGGTACC3900.23314482478269705No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC3810.22776455959540404No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3470.20743911333229711No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC3270.195482968471646No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGTGGGTACC3260.19488516122861343No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTTACCTGTTACC3140.18771147431222276No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3130.1871136670691902No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCGGGTACC2920.1745597149655065No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2900.17336410047944142No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACTGCTACC2870.17157067875034374No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC2750.16439699183395307No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGGGGGTACC2740.1637991845909205No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2720.16260357010485538No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA2690.16081014837575772No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC2660.15901672664666006No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC2640.15782111216059494No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2570.15363646145936705No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2530.1512452324872368No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCACCTACC2400.14347373832781357No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCCTCCGGTACC2360.14108250935568337No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC2350.1404847021126508No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGCGCCTACC2250.13450662968232524No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2170.12972417173806478No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2110.12613732827986945No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2060.12314829206470665No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC2040.12195267757864155No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC2010.12015925584954387No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCCTCAGGTACC2000.11956144860651131No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1910.11418118341921832No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTCCAGCTACC1880.11238776169012064No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1870.11178995444708809No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1840.10999653271799041No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC1820.10880091823192531No Hit
TTACCGCGGCTGCTGGCACGAATTTAGCCGGGGCTTCTTCTTCTGCTACG1800.10760530374586018No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCATTAACCCAGCTA1730.10342065304463229No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1690.10102942407250205No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC1680.10043161682946951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)