FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122651
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2081716.972548124352837No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1763214.375749076648376No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC89227.274298619660663No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC63505.17729166496808No Hit
TTACCGCGGCTGCTGGCACCTGAGCCAGGATCAAACTCTTGTAGAACCAT27712.2592559375789842No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC26132.1304351370963137No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC24351.9853079061727994No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT16701.3615869418105029No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC14881.2131984247988195No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT12571.0248591532070672No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC12321.0044761151560118No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC10480.8544569551002438No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC6710.5470807412903279No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA6380.5201751310629347No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT6380.5201751310629347No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC6370.5193598095408924No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT6130.4997920930118792No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC6040.49245419931349926No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC5780.47125583974040164No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC5540.4516881232113884No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC5490.4476115156011773No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5420.4419042649468818No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC5130.4182599408076575No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC5120.41744461928561527No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC4910.40032286732272876No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC4470.3644487203528712No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGACTACC4420.36037211274266007No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCCGGTACC4330.3530342190442801No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3690.3008536416335782No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTCCGACTACC3140.2560109579212563No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTGTTTTTCAGGTACC3010.24541177813470744No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC2900.23644324139224301No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2680.21850616790731425No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2500.20383038051055435No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC2440.19893845137830105No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2350.19160055767992107No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2010.1638796259304857No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC1940.15817237527619016No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCATAAGGTACA1910.15572641071006352No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC1900.1549110891880213No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCAGGTACT1870.15246512462189465No Hit
CTACCGCGGCTGCTGGCACCTGAGCCAGGATCAAACTCTTGTAGAACCAT1860.15164980309985243No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC1840.15001916005576799No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCCTCAGGTACC1830.14920383853372576No Hit
TTACCGCGGCTGCTGGCACCTGAGCCAGGATCAAACTCTTGTAGAACCCT1750.14268126635738804No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCATACAGTACC1750.14268126635738804No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA1730.1410506233133036No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTAGGGTACA1720.1402353017912614No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1710.13941998026921917No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCCCTTTCTGGTATGGTACC1700.13860465874717695No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1700.13860465874717695No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGTGGCTTATTCCTCAAGTACC1660.1353433726590081No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCGTAAAGTACA1590.12963612200471256No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC1590.12963612200471256No Hit
TTACCGCGGCTGCTGGCACCTGAGCCAGGATCACACTCTTGTAGAACCAT1570.12800547896062814No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1570.12800547896062814No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTTTTCTTTGGGTTAG1500.12229822830633261No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1470.11985226374020595No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATTCCGGTACC1390.11332969156386821No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCAGGTACT1340.10925308395365713No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC1320.1076224409095727No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC1300.10599179786548825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)