FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135263
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3339124.685982123714542No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC127049.392073220318935No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC73815.456776797793927No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC40933.0259568396383343No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC33542.479613789432439No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC32992.438952263368401No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC25901.9147882273792538No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC21921.6205466387703953No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT14091.0416743677132696No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC13731.0150595506531719No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC12940.9566548132157352No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC12500.9241255923645048No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGTTGGTACC12490.923386291890613No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA12100.8945535734088406No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC11470.8479776435536694No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC11230.830234432180271No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA10680.7895729061162328No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC8780.649105816076828No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8300.6136193933300311No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC6190.4576269933389028No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5980.4421016833871791No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC4850.3585607298374278No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4570.33786031656846294No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC4480.3312066123034385No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3980.2942415886088583No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3920.28980578576550864No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC3800.28093418007880944No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTAGGTACC3300.24396915638422922No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC3260.2410119544886628No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3040.22474734406304755No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC2970.2195722407458063No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC2490.18408581799900936No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2480.18334651752511774No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2480.18334651752511774No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2420.1789107146817681No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC2390.17669281326009328No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2380.1759535127862017No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2350.17373561136452687No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC2200.16264610425615283No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC2100.15525309951723681No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGGTTAGATACC2030.15007799619999557No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC1960.14490289288275432No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1830.1352919867221635No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1750.12937758293103066No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC1630.1205059772443314No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1610.11902737629654819No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1580.11680947487487339No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTTAGATACC1500.11089507108374057No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGATACC1490.11015577060984895No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC1480.10941647013595736No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCCGGTAAGATACC1380.10202346539704132No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATAAC1370.1012841649231497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)