FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150003
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG4179627.863442731145376No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG4048026.986126944127786No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG3240521.602901275307826No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG26231.748631694032786No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG11450.7633180669719939No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG8760.5839883202335954No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG7610.5073231868695959No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG5820.3879922401551969No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG5770.3846589734871969No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG4660.3106604534575975No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG3650.24332846676399805No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCATGCCTAATACATG3390.2259954800903982No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG3340.22266221342239823No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG3210.2139957200855983No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGTAGGCCTAACACATG2790.18599628007439853No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG2740.18266301340639854No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2700.17999640007199855No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAATACATG2380.15866349339679872No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2330.15533022672879876No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG2160.14399712005759885No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG2040.1359972800543989No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG1970.13133070671919894No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACGTG1920.12799744005119898No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTGGGCCTAACACATG1880.12533082671679902No Hit
AGAGTTTGATCCTGGCTCAGAGTGAGCGCTGGCGGTAGGCCTAACACATG1800.11999760004799903No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG1750.11666433337999907No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG1730.11533102671279907No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACACG1710.11399772004559909No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCCGGCGGTAGGCCTAACACATG1600.10666453337599914No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG1590.10599788004239914No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG1580.10533122670879916No Hit
AGAGTTTGATCCTGGCTCGGAGTGAACGCTGGCGGTAGGCCTAACACATG1510.1006646533735992No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG1510.1006646533735992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)