FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150003
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3879525.86281607701179No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3034520.22959540809184No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2470516.46967060658787No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC24361.6239675206495872No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT20101.3399732005359892No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA17841.1893095471423905No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC16081.0719785604287915No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC11780.7853176269807937No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT9040.6026546135743951No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT8350.5566555335559955No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC8000.5333226668799957No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC7220.4813237068591962No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC6550.43665793350799653No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACC5390.35932614681039715No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC5000.33332666679999734No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4850.3233268667959974No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC4440.2959940801183976No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC3690.24599508009839804No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC3620.24132850676319806No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTGTCGGTAAC2740.18266301340639854No Hit
TTACCGCGGCTGCTGGCACGCAGTTAGCCGGTGCTTATTCTTTGGGTACC2620.1746631734031986No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGAGCTTATTCTCCCGGTACT2620.1746631734031986No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTAAC2420.1613301067311987No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGGTACT2370.15799684006319875No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2320.15466357339519876No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC2280.15199696006079877No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTAGGTACC2090.1393305467223989No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTGGGTACC2060.1373305867215989No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGCGCTTATTCTTTGGGTACC1890.125997480050399No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACC1770.11799764004719905No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTAAT1750.11666433337999907No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC1730.11533102671279907No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTCTGGGTACC1670.11133110671119911No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGGTACT1600.10666453337599914No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCGGGTACC1600.10666453337599914No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGGTACT1530.10199796004079918No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1530.10199796004079918No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTCCCGGTACT1520.10133130670719918No Hit
TTACCGCGGCTGCTGGCACGAGGTTAGCCGGTGCTTATTCTTTGGGTACC1510.1006646533735992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)