FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164759
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG7562145.89794791179844No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG3635422.064955480428992No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1937211.757779544668272No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG8730.5298648328771114No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG8030.48737853470827086No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG7820.4746326452576187No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCATGCCTAATACATG7050.42789771727189413No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG6250.37934194793607634No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG6200.37630721235258774No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG5440.3301792314835609No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGTAGGCCTAACACATG4000.24277884667908886No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG3800.23063990434513443No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG3520.21364538507759817No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG3410.20696896679392326No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAATACATG3380.20514812544383007No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAATACATG3370.20454117832713237No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAATACATG3060.18572581770950297No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG2800.1699451926753622No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2720.16508961574178044No Hit
AGAGTTTGATCCTGGCTCAGAACGAGCGCTGGCGGCATGCCTAATACATG2610.15841319745810548No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACACG2370.14384646665736014No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGTAGGCCTAACACATG2270.13777699549038291No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG2250.13656310125698748No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACGTG2180.13231447144010341No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCCGGCGGCATGCCTAATACATG2130.1292797358566148No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCACGCCTAATACATG2100.12745889450652165No Hit
AGAGTTTGATCCTGGCTCAGAACGGACGCTGGCGGCATGCCTAATACATG1980.120175529106149No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCCAATACATG1740.10560879830540365No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG1690.10257406272191504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)