FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164759
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT5591133.935020241686345No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3123718.959207084286746No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC141688.599226749373328No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT29491.7898870471415826No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT17981.0912909158225044No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC16561.0051044252514278No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT16280.9881099059838916No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACC13070.7932798815239228No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC8060.489199376058364No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC7520.456424231756687No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC5720.3471737507510971No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5670.3441390151676084No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC5500.3338209141837472No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC4720.2864790390813248No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGGTACT4680.28405125061453396No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC4530.2749470438640681No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGAGCTTATTCTCCCGGTACT4200.2549177890130433No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC4020.2439927409124843No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGGTACT3820.23185379857852986No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTAAT3780.229426010111739No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC3590.21789401489448224No Hit
TTACCGCGGCTGCTGGCACGCAGTTAGCCGGTGCTTATTCTTTGGGTACC2990.1814771878926189No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTAAC2880.17480076960894397No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGGTACT2740.16630350997517587No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTCCCGGTACT2600.15780625034140774No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGGTACT2580.1565923561080123No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTGTCGGTAAC2350.1426325724239647No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGGTACT2330.14141867819056925No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTAGGTACC2270.13777699549038291No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT2170.1317075243234057No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2150.13049363009001025No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTCCT2140.12988668297331254No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACTG2090.12685194738982392No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGGTACT2090.12685194738982392No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC2080.1262450002731262No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCGCCCGGTACT1930.11714079352266038No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTCCCGGTACT1890.1147130050558695No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGGTACT1880.11410605793917175No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGGTACT1860.1128921637057763No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTGGGTACC1850.11228521658907858No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGGTACT1800.10925048100558997No Hit
TTACCGCGGCTGCTGGCACGGCGTTAGCCGGAGCTTATTCTCCCGGTACT1660.10075322137182187No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)