FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163171
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG8418851.59495253445772No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG2281313.981038297246448No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1706510.45835350644416No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG20481.2551249915732576No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG18651.1429727096113893No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG14020.8592213077078646No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG11500.7047820997603741No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG8160.5000888638299698No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG7810.4786389738372627No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG6470.39651653786518437No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG3990.24452874591686025No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG3960.2426901839174853No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG3740.2292073959220695No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG3440.2108217759283206No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG3380.20714465192957082No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG3250.19917754993227965No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG2890.17711480593978096No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG2740.16792199594290652No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG2660.1630191639445735No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG2640.16179345594499023No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG2590.15872918594603208No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAATACATG2570.1575034779464488No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG2530.1550520619472823No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG2450.15014922994894925No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG2420.14831066794957437No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAGCACATG2390.1464721059501995No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG2000.12257079995832591No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG1980.12134509195874266No Hit
AGAGTCTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1910.11705511396020125No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCCAACACATG1880.11521655196082636No Hit
AGAGTTCGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1870.11460369796103476No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG1830.11215228196186822No Hit
GGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1800.11031371996249333No Hit
AGGGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1740.10663659596374356No Hit
AGAGTTTGACCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG1700.10418517996457705No Hit
GAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGC1670.10234661796520216No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG1670.10234661796520216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)