FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163171
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC6370439.041251202725974No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2139713.1132370335415No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT125437.6870277193864105No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC52643.2260634549031386No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC20471.2545121375734658No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC18981.163196891604513No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC18871.156455497606805No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC17561.0761716236341017No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC12970.7948716377297436No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT10790.6612694657751683No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC7890.4835418058355958No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC7410.45412481384559755No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC5810.3560681738789368No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC5710.3499396338810205No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC5020.30765270789539806No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4690.2874285259022743No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC4480.2745585919066501No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC4270.26168865791102586No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3910.23962591391852717No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3650.2236917099239448No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT3300.2022418199312378No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC2840.17405053594082281No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTGGGTACC2720.16669628794332325No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC2650.16240630994478186No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGCGCTTATTCTGTCGGTAAC2620.16056774794540696No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCCTATTCTGTCGGTAAC2580.15811633194624045No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATCCTGTCGGTAAC2480.15198779194832415No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC2460.15076208394874088No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCGTCGGTAAC2440.14953637594915764No Hit
TTACCGCGGCTGCTGGCATAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2430.148923521949366No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACC2380.14585925195040786No Hit
TACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAACG2330.1427949819514497No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2300.1409564199520748No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC2290.1403435659522832No Hit
TTACAGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2030.12440936195770082No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC1910.11705511396020125No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC1870.11460369796103476No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTAAC1850.11337798996145147No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTACC1830.11215228196186822No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC1830.11215228196186822No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC1750.10724944996353518No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAGC1700.10418517996457705No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)