FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191056
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT6435933.68593501381794No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3200816.753203249309102No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2879615.072020768779835No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT34651.8136043882421908No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT18840.9860983167238925No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC17350.9081107109957289No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT17030.8913616950004187No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC15790.8264592580185914No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACC12100.6333221673226699No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC10610.5553345615945063No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC8940.4679256343689808No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC8180.4281467213801189No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC5520.2889205259191023No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT5270.27583535717276614No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC5270.27583535717276614No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGGTACT5160.2700778829243782No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGGTACT4690.24547776568126625No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTGGGTACC4060.21250314044049912No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTGTCGGTAAC3720.19470731094548194No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGAGCTTATTCTCCCGGTACT3670.19209027719621471No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTAAT3610.18894983669709406No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC3460.18109873544929236No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC3260.17063060045222342No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC3250.17010719370236999No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGGTACT3230.1690603802026631No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC3110.16277949920442175No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC2930.1533581777070597No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGGTACT2920.15283477095720627No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC2780.145507076459258No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC2690.140796415710577No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTAAC2590.13556234821204255No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC2460.12875806046394772No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACTG2340.12247717946570638No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGGTACT2260.11828992546687883No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTCCCGGTACT2070.10834519721966333No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGGTACT2000.10468134997068922No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGGTACT2000.10468134997068922No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT1960.10258772297127544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)