FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144846
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG4603731.78341134722395No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG3622725.010701020394073No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1542510.64924126313464No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG81805.647377214420833No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG31062.1443464092898665No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG30002.071165237562653No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG10230.7062673460088646No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG8450.5833782085801472No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG8150.5626665562045207No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG7790.5378125733537689No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG7010.48396227717713985No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5660.39075984148682047No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG4730.3265537191223783No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG4330.29893818262154287No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG3310.2285185645444127No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG2460.16983554948013754No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG2430.16776438424257487No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG2390.16500283059249135No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2360.16293166535492867No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG2210.15257583916711542No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG2120.14636234345442745No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG2070.14291040139182304No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2010.13876807091669774No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG1960.13531612885409333No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1910.1318641867914889No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG1830.1263410794913218No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG1790.12357952584123828No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG1760.12150836060367562No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG1660.11460447647846678No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG1630.11253331124090413No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCTTAACACATG1510.10424865029065353No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG1450.1001063198155282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)