FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141351
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG4984635.263988227886614No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2609518.461135754257132No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG123488.735700490268904No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG99177.015868299481433No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG72085.09936257967754No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG31922.2582082900014857No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG14181.0031764897312363No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG7890.5581849438631492No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG6900.48814652885370463No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG5200.36787854348395127No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG5050.35726666242191424No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG4910.34736224009734634No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG4620.32684593671074136No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG4430.31340422069882773No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG4420.3126967619613586No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGTAGGCCTAACACATG3520.24902547558913624No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG3250.22992408967746955No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG3220.22780171346506217No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTGGGCCTAACACATG2460.17403484941740774No Hit
AGAGTTTGATCCTGGCTCAGAGTGAGCGCTGGCGGTAGGCCTAACACATG2380.16837517951765463No Hit
AGAGTTTGATCCTGGCTCGGAGTGAACGCTGGCGGTAGGCCTAACACATG2290.1620080508804324No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGTAGGCCTAACACATG2220.15705583971814843No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG2210.1563483809806793No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGCGCCTAACACATG2170.15351854603080275No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACGCATG2160.15281108729333362No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACGTG2030.14361412370623483No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACACG1940.1372469950690126No Hit
AGAGTTTGATCCTGGCTCAGAGTGGACGCTGGCGGTAGGCCTAACACATG1750.12380527905709898No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAATACATG1720.12168290284469158No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1660.11743815041987675No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1610.11390085673253109No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG1590.11248593925759282No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCCGGCGGTAGGCCTAACACATG1480.10470389314543228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)