FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157466
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG5404934.324235072968136No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2273514.43803741760126No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1855011.780320831163554No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG77434.917251978204819No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG48433.0755845706374707No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCCTAACACATG43012.731383282740401No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG36862.3408227807907735No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG23901.5177879669261936No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG11690.7423824825676654No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG10430.6623652089974978No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG9130.5798077045203409No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTCACACATG7300.4635921405255738No Hit
AGAGTTTGATCCTGGCTCAGAAGGAACGCTAGCTATATGCTTAACACATG7030.446445581903395No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG6810.43247431191495306No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG5810.36896853924021694No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGCGTGCTTAAGACATG5450.3461064610773119No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG5170.32832484472838586No Hit
AGAGTTTGATCCTGGCTCAGGATAAACGCTGGCGGTGTACATAAGACATG4930.31308345928644915No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG4690.2978420738445125No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG4430.28133057294908104No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG3810.24195699389074468No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG3430.21782480027434495No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGTATGCTTTACACATG3270.20766387664638716No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG3090.19623283756493465No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3080.1955977798381873No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG3040.19305754893119784No Hit
AGAGTTTGATCCTGGCTCAGGATGAATGCTGGCGGCGTGCCTAACACATG3000.1905173180242084No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2890.18353168302998743No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2770.1759109903090191No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2550.16193972032057716No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGTTGGCGGCGCGCCTAACACATG2450.15558914305310353No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG2040.12955177625646172No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG1960.12447131444248281No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1870.11875579490175656No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1870.11875579490175656No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGTTAGCGGCGCGCCTAACACATG1820.11558050626801976No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGACAGGCTTAACACATG1810.1149454485412724No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1790.11367533308777769No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1710.1085948712737988No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCTTAACACATG1710.1085948712737988No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1670.10605464036680934No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAACACATG1590.10097417855283045No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTCATACATG1590.10097417855283045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)