FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157466
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2451915.570980402118554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1954812.414108442457419No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC79415.042993408100797No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC70094.451119606772255No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT62583.974191253984987No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC37022.3509837044187316No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC34422.1858686954644178No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT34232.1738025986562177No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC33812.1471301741328284No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC29171.852463388922053No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC27991.7775265771658646No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC24121.5317592369146356No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC14710.9341699160453685No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC11010.6991985571488448No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC9890.6280720917531404No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC9850.6255318608461509No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC9050.574727242706362No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC8450.5366237791015204No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC7380.46867260233955266No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6590.41850304192651117No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT5960.37849440514142735No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACC5890.3740490010541958No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC5530.35118692289129083No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC5410.3435662301703225No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC5290.3359455374493542No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC5060.32133920973416485No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC4880.3099081706527123No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4730.3003823047515019No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC4560.28958632339679674No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTATTACTCTGGTACCG4410.2800604574955864No Hit
TTACCGCGGCTGCTGGCACATATTTAGCCAGGGCTTCCTCCTGTGATGGT4340.27561505340835485No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4280.27180470704787063No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT4180.26545412978039706No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTTTCTGCAGGTACC3900.2476725134314709No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3520.2235403198150712No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC3370.21401445391386076No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCACTTCTTCTGTTGGTACC3110.19750295301842938No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3060.19432766438469257No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3010.19115237575095578No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2930.1860719139369769No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC2820.1790862789427559No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTCAGGTACA2640.16765523986130337No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGTTACC2580.1638448935008192No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC2530.16066960486708243No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCATGAGTTACC2480.1574943162333456No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2350.1492385657856299No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2310.14669833487864048No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2290.14542821942514575No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2260.14352304624490364No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2170.13780752670417742No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC2130.13526729579718796No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2110.13399718034369323No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC2110.13399718034369323No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGATCCTTATTCATAAGTTACT2010.12764660307621964No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC1940.12320119898898811No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC1900.12066096808199865No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTGCTAAAGAGGTACC1880.11939085262850392No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC1870.11875579490175656No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT1870.11875579490175656No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCAGGGCTTGCTTCTTGGTTACA1840.11685062172151449No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1790.11367533308777769No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTATTCCGGTACC1770.11240521763428296No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1750.11113510218078823No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC1740.11050004445404087No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC1710.1085948712737988No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC1630.1035144094598199No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT1610.10224429400632518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)