FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163680
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC4338326.50476539589443No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1812011.070381231671554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC138378.453690127077223No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC73254.47519550342131No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT43972.686339198435973No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT38502.3521505376344085No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC31841.9452590420332354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC27551.6831622678396871No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC26811.637952101661779No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT22971.4033479960899315No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC19621.1986803519061584No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC16561.0117302052785924No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC12820.7832355816226784No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC12450.7606304985337243No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC11800.7209188660801563No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC10740.656158357771261No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8880.5425219941348974No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC7730.4722629521016618No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC5030.3073069403714565No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC4450.27187194525904207No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCGAGTAAC4400.2688172043010753No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC4330.2645405669599218No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC4170.25476539589442815No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC3780.23093841642228738No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC3420.2089442815249267No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3410.20833333333333334No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC3150.19244868035190615No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT2930.1790078201368524No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2880.17595307917888564No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2810.17167644183773217No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC2770.16923264907135874No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC2500.15273704789833822No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC2470.15090420332355817No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC2400.1466275659824047No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC2220.13563049853372436No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGCGCTTATTCTGTCGGTAAC2210.13501955034213098No Hit
TTACAGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2190.13379765395894427No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT2160.13196480938416422No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC2060.1258553274682307No Hit
TTACCGCGGCTGCTGGCATAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2000.12218963831867058No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC1900.11608015640273706No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC1900.11608015640273706No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC1860.11363636363636363No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTACC1820.11119257086999024No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1810.11058162267839687No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTACCCACTACC1740.1063049853372434No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT1690.10325024437927664No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTCTCGGTAAC1680.10263929618768329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)