FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141864
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2940220.725483561721084No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC1959013.80900016917611No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC62654.416201432357751No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC53523.7726273050245305No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC34212.411464501212429No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC28131.982885016635651No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT24321.714317938307111No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT18921.3336716855579993No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC18771.3230981785371905No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT17641.2434444256470987No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCTACAGTACC15891.1200868437376643No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC15471.0904810240794No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC14741.0390232899114644No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCACTACC11630.8197992443466983No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCTTCAGGTACA11330.7986522303050809No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATATAGTACC10740.7570631026899002No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC10580.7457846952010376No Hit
TTACCGCGGCTGCTGGCACACTGAGCCAGGATCAAACTCTTGTAGAACCA9330.6576721366942988No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCAGGTACT9220.6499182315457057No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACAGTACC8880.6259516156318727No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT8470.5970506964416624No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC6700.47228331359612025No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC6410.4518412000225568No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACC5820.4102520724073761No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC5670.39967856538656743No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC5470.3855805560254892No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4450.3136807082839903No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC4440.31297580781593637No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTTACTGGGTACC3780.2664523769243783No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC3390.23896125867027576No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC3150.2220436474369819No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCGCTGGGATAC2970.2093554390120115No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2770.1952574296509333No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTTTTCTGCAGGTACC2650.18679862403428635No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATTCCGGTACA2430.1712908137371003No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCAGGTACT2350.16565160999266904No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGATGCTTATTCTTACCATACT2300.1621271076523995No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2230.15719280437602212No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTACTCTTACGGTACC2070.14591439688715954No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTAAC1930.13604579033440478No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCCCCAGATACT1890.13322618846218914No Hit
TTACCGCGGCTGCTGGCACAGAGTTTGCCGGTGCTTATTCTTAAGGTACT1850.1304065865899735No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTAAT1720.12124288050527265No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1700.11983307956916482No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTACGGTACC1640.11560367676084138No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCAGGTACT1630.11489877629278746No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT1620.11419387582473355No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCAGGTACT1610.11348897535667965No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC1590.11207917442057182No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGTTACC1530.10784977161224835No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1430.10080076693170924No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)