FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101052
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG3038630.069667102086058No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG1647516.303487313462377No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG94889.3892253493251No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG92619.164588528678303No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG89188.825159323912441No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG25462.519494913509876No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG6540.6471915449471559No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG6080.601670427106836No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCAGTATGGATAAGGCATG5590.5531805407117129No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG5430.5373471084194276No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG4750.47005502117721565No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCTACAGGCTTAACACATG3040.300835213553418No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2700.26718916993231207No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2560.25333491667656255No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACGTG2460.24343902149388436No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG2410.23849107390254526No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2350.23255353679293828No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2050.20286585124490364No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG1880.18604282943435063No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGCGTCTTAAGCATG1690.167240628587262No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1600.1583343229228516No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGTATGGATAAGGCATG1500.14843842774017338No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1300.12864663737481694No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAACACATG1290.1276570478565491No Hit
AGAGTTTGATCCTGGCTCGGAACGAACGCTGGCGGCATGCCTAACACATG1210.11974033171040653No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1190.11776115267387087No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCCTAACACATG1180.11677156315560305No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG1080.10687566797292483No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG1070.105886078454657No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1040.10291730989985355No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCATGCCTAACACATG1020.10093813086331789No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)