FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154922
Sequences flagged as poor quality0
Sequence length300
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG5567835.939375944023446No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG3803424.55041892048902No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG1654610.680213268612594No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG52203.3694375233988714No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG50333.248731619782858No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG9780.6312854210505932No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG8570.5531816010637611No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG7430.4795961838860846No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG6380.411820141748751No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG6070.39181007216534774No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG5600.361472224732446No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG3480.22462916822659143No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG3470.2239836821109978No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG2790.18009062625062935No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGCAGGCCTAACACATG2710.1749267373258801No Hit
AGAGTTTGATCCTGGCTCAGATCGAACGCTGGCGGCAGGCCTAACACATG2700.17428125121028648No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG2690.1736357650946928No Hit
AGAGTTTGATCCTGGCTCAGATTGAGCGCTGGCGGCAGGCCTAACACATG2680.17299027897909916No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG2680.17299027897909916No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCGGGCCTAACACATG2620.16911736228553723No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG2530.16330798724519435No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGTAGGCCTAACACATG2460.15878958443603877No Hit
AGAGTTTGATCCTGGCTCAGGTTGAACGCTGGCGGCAGGCCTAACACATG2330.15039826493332129No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAATACATG2320.14975277881772764No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACGCATG2160.13942500096822918No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACACG2070.13361562592788628No Hit
AGAGTTTGATCCTGGCTCGGATTGAACGCTGGCGGCAGGCCTAACACATG2070.13361562592788628No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCCGGCGGCAGGCCTAACACATG2050.132324653696699No Hit
AGAGTTTGATCCTGGCTCAGACTGAACGCTGGCGGCAGGCCTAACACATG1970.12716076477194976No Hit
AGAGTTTGATCCTGGCTCAGATTGGACGCTGGCGGCAGGCCTAACACATG1890.12199687584720054No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG1820.11747847303804496No Hit
AGAGTTTGATCCTGGCTCAGAGTGAGCGCTGGCGGTAGGCCTAACACATG1700.10973263965092112No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACGTG1640.10585972295735918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)