FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154922
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3902325.188804688811146No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC3195420.625863337679608No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT126898.19057332076787No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC57243.6947625256580734No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC35332.280502446392378No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC28971.8699732768748145No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC23851.5394843856908638No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC21361.378758342908044No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC17231.112172577167865No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC12400.8004027833361304No Hit
TNACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC10230.6603322962523076No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT10010.6461316017092472No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC8440.5447902815610436No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC7410.4783052116548973No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC5860.37825486373788103No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC4520.2917597242483314No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTACTCTTTGGGTACC4260.27497708524289644No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTCTTCTTTGGGTACC4180.2698131963181472No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC4040.26077639069983605No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTCTTCTGTCGGTAAC3810.24593021004118198No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGGTACT3640.2349569460760899No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTACTCTGTCGGTAAC3340.2155923626082803No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT3270.21107395979912472No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC3240.20913750145234375No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTTGGGTACC3030.19558229302487704No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTCGGTACC2960.19106389021572148No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2930.18912743186894052No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTCGGTAAC2780.17944514013503568No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTAGGTAAC2540.16395347336078803No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTAAC2230.14394340377738474No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACC2220.1432979176617911No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTGTCGGTAAC2110.1361975703902609No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTGGGTACC1920.12393333419398148No Hit
TTACCGCGGCTGCTGGCACGCAGTTAGCCGGTGCTTATTCTTTGGGTACC1860.12006041750041957No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTAGGTACC1780.11489652857567033No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTCAC1760.11360555634448302No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTTTGGGTACC1750.11296007022888939No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA1700.10973263965092112No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTGTCGGTAAC1680.10844166741973381No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCGTCGGTAAC1620.10456875072617187No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGCCGGTAAC1610.10392326461057823No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTCTGGGTACC1610.10392326461057823No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGCGCTTATTCTTTGGGTACC1600.10327777849498457No Hit
TTACCGCGGCTGCTGGCACAGGGTTAGCCGGTGCTTATTCTGTCGGTAAC1570.1013413201482036No Hit
TTACCGCGGCTGCTGGCATAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC1560.10069583403260997No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATCCTGTCGGTAAC1560.10069583403260997No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAAC1550.1000503479170163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)