FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116754
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2912324.943899138359285No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC108499.292186991452112No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC90077.714510851876595No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC79626.819466570738476No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC33072.832451136577762No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC19281.6513352861572195No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC18021.543416071397982No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC11510.985833461808589No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGGTACC10120.8667797248916526No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC8490.7271699470681947No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC7750.6637888209397537No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC6800.5824211590181064No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6230.5336005618651181No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC5760.49334498175651365No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCCAGGTACC5580.4779279510766226No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGCGGGTACC5440.4659369272144852No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC5280.4522328999434709No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC5140.4402418760813334No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC4900.41968583517481195No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTCAGTAATTACC4830.4136903232437433No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC4800.41112081813042806No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC3890.33317916302653444No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCACCTACC3590.3074841118933827No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTACTTACC3250.27836305394247735No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC3220.2757935488291621No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC3200.27408054542028537No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGCTACC3140.26894153519365505No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2960.253524504513764No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC2530.21669493122291314No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2380.20384740565633727No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGCCTTTCTAAGTAATTACC2220.19014337838532297No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2130.18243486304537745No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTCCTTGGGTACC2070.1772958528187471No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2060.17643935111430872No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1900.16273532384329445No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC1860.15930931702554088No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCCTGGAGTACC1850.15845281532110247No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1840.1575963136166641No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCATCTACC1830.1567398119122257No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGCTACC1830.1567398119122257No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGCTACC1760.15074429998115696No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAGTTACC1740.14903129657228018No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCAGGTCCTTATTCGTTGGTTACC1730.14817479486784177No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGCTACC1670.14303578464121144No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1670.14303578464121144No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC1570.13447076759682752No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTCTCTGGTAAGCTACC1490.12761875396132039No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC1470.1259057505524436No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC1430.12247974373469003No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCCTTCTGGTAAGCTACC1350.1156277300991829No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGTTAAGCTACC1270.10877571646367576No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC1240.1062062113503606No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTCCTGGTAAGCTACC1210.1036367062370454No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1200.10278020453260701No Hit
TTACCGCGGCTGCTGGCACGTAGCTAGCCGTCCCTTTCTGGTAAGCTACC1190.10192370282816864No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTAAC1180.10106720112373023No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)