FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168283
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC2411214.328244683063648No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2308713.71915166713215No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC107516.388642940760504No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC56353.348525994901446No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC51053.0335803378832087No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC43992.614048953251368No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC32311.9199800336338193No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC27881.65673300333367No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA23491.3958629213883755No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC21631.2853348228876358No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC17741.0541765953780238No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC17591.0452630390473192No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCCTCGAGTCCT15940.9472139194095661No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTAAGTAATTACC12380.7356655158275048No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC12060.7166499289886679No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC11070.657820457206016No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC11040.6560377459398751No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC10420.6191950464396285No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGGGCTGATTCGGGGGGTACC9180.5455096474391353No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC8140.4837089902129152No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGGTGGTACC6890.4094293541237083No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC6530.3880368189300167No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC6530.3880368189300167No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTATTCGAGTCTT6340.37674631424445726No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACC6310.37496360297831627No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC5450.32385921334894197No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGGATACC5100.30306091524396406No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTGTACCTACC4890.2905819363809773No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGGGCTTCTTCTGCAGGTACC4890.2905819363809773No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC4820.2864222767599817No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC4670.2775087204292769No Hit
TTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTATTCGTATGGTACC4340.25789889650172626No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4240.2519565256145897No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC4170.2477968659935941No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTATTACTCTGGTACCG3770.22402738244504794No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCCGGTACC3660.2174907744691977No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACAGTACC3560.21154840358206117No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC3530.2097656923159202No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCCTCAGGTACC3490.2073887439610656No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3390.20144637307392904No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC3190.18956163129965595No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATCAGGTACC3180.18896739421094227No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC3170.18837315712222863No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTTCAGGTACC3160.18777892003351496No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGGTACC3120.18540197167866035No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC3100.18421349750123306No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTGCGGTACC3090.1836192604125194No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCGTTAGGTACC3080.18302502332380574No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC3040.1806480749689511No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCCTCCTATGGTACC2970.17648841534795554No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC2880.17114028154953262No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACTGCTACC2870.17054604446081897No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGAAGGTACC2730.1622267252188278No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAAGTACC2710.1610382510414005No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC2520.14974774635584104No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCCTCTGCAGGTACC2450.14558808673484547No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGTGGGTACC2420.1438053754687045No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTCTGCAGGTACC2390.14202266420256354No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC2370.14083419002513622No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC2290.13608029331542698No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGACCTTACAAATGGTACCGT2260.13429758204928602No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC2250.13370334496057235No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTCCAGGTACC2200.1307321595170041No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC2160.12835521116214946No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2140.12716673698472217No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC2010.11944165483144466No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGATACC2000.118847417742731No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTACGGTACC1920.11409352103302174No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCTTCCGGTACA1890.11231080976688079No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC1850.10993386141202616No Hit
CTACCGCGGCTGCTGGCACGGAATTAGCCGGTCCTTTTTCTGAAGGTACA1820.10815115014588522No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC1810.10755691305717155No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC1770.10517996470231694No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1740.10339725343617596No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT1740.10339725343617596No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTTAGTTACC1740.10339725343617596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)