FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154909
Sequences flagged as poor quality0
Sequence length300
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG5540635.76680502746774No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG4335927.98998121477771No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG1696710.952882014602121No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG24491.580928157821689No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG17801.1490617071958376No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGGATAAGGCATG13820.8921366737891278No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG6950.4486504980343298No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG4940.318896900761092No Hit
AGAGTTTGATCCTGGCTCGGGATGAACGCTGGCGGCGTGCCTAATACATG4210.2717724599603638No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG3460.2233569385897527No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3250.20980059260598155No Hit
AGAGTTTGATCCTGGCTCAGGATGAGCGCTGGCGGCGTGCCTAATACATG3160.20399073004150822No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG3060.1975353271920934No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG2740.17687803807396602No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACGTG2730.1762324977890245No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2570.16590385322996082No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCCAATACATG2510.16203061152031192No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG2430.15686628924078005No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2360.1523475072461897No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCCTAATACATG2260.14589210439677489No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2200.142018862687126No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTGATACATG2090.1349179195527697No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1990.12846251670335487No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1930.12458927499370599No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCTTAACACATG1900.12265265413888154No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1890.12200711385394006No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAGTACATG1800.11619725128946672No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACACG1800.11619725128946672No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGCGTGCCTAATACATG1780.11490617071958376No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAATACATG1710.11038738872499339No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCCAATACATG1630.10522306644546153No Hit
AGAGTTTGATCCTGGCTCAGGACGGACGCTGGCGGCGTGCCTAATACATG1620.10457752616052005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)