FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144534
Sequences flagged as poor quality0
Sequence length300
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG4541931.424439924170088No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG2193815.178435523821385No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG1671511.56475293010641No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG62454.320782653216544No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG55733.8558401483387987No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG31662.190488051254376No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG30342.099160059224819No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG21791.507603747215188No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG17441.206636500754148No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG14190.9817759143177384No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATG14070.9734733695877786No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGCGCCTAACACATG12330.8530864710033624No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG11330.783898598253698No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGACAGAATGCTTAACACATG9440.6531335187568323No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG7430.5140658945300067No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCCTAACACATG6170.4268891748654296No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCCGGCGGTGTGCCTAATACATG5210.36046881702575173No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAATACATG3870.2677570675412014No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATG3700.25599512917375844No Hit
AGAGTTTGATCCTGGCTCAGAATCAACGCTGGCGGCGTGCCTCAGACATG3550.24561694826130878No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAACACATG3390.23454688862136244No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTTACACATG3350.23177937371137589No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAATACATG3150.217941799161443No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCGTGCCTAACACATG2920.20202858842902016No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGGATTAGGCATG2910.2013367097015235No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG2870.19856919479153695No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCGTGCCTAATACATG2800.1937260436990604No Hit
AGAGTTTGATCCTGGCTCGGGACGAACGCTGGCGGCGTGCCTAATACATG2780.19234228624406713No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCTTAACACATG2770.1916504075165705No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGCAGGCCTAACACATG2600.17988846914912754No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGTTGGCGGCGTGGATTAGGCATG2440.16881840950918123No Hit
AGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCGTGCATAACACATG2340.16189962223421478No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACACG2260.15636459241424164No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCGTGCTTAACACATG2220.15359707750425505No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCATGCTTAACACATG2160.1494458051392752No Hit
AGAGTTTGATCCTGGCTCAGGGCGAACGCTGGCGGCGTGCCTAATACATG2010.1390676242268255No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCATGCCTAACACATG2010.1390676242268255No Hit
AGAGTTTGATCCTGGCTCAGGACGAGCGCTGGCGGCGTGCCTAATACATG1520.10516556657948996No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGTGGCGTGCTTAACACATG1500.10378180912449665No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACGTG1480.10239805166950336No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCCGGCGGCGTGCCTAATACATG1460.10101429421451008No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGTTGGCGGCGTGGATTAGGCATG1450.10032241548701343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)