FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144534
Sequences flagged as poor quality0
Sequence length300
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2707818.734692183154138No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC96286.6614083883376916No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC69374.799562732644222No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC55863.864834571796256No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT41162.8477728423761883No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC30542.112997633774752No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC30072.0804793335824097No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC27681.9151203177107117No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC24531.6971785185492687No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC23851.650130765079497No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC22661.567797196507396No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCACGTACC20321.4058975742731814No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAGGTACC18541.2827431607787787No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC16721.1568212323743894No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC16211.1215354172720606No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC11970.8281788368134834No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCAGGTACC10910.754839691698839No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTACTTCTTCTGCAGGTACC10890.7534559342438457No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACC7900.5465841947223491No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTGTAGGTACC7820.5410491649023759No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC6970.48223947306516113No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC6550.45318056651030214No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCCAAATACC6390.44211050687035575No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC6350.4393429919603692No Hit
TTACCGCGGCTGCTGGCACGTATTTAGCCGTCGCTTCTTTAGCAGGTACA6320.43726735577787923No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTACCACTACC6130.42412165995544304No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTATAGTACC4870.33694494029086586No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGATACC4800.3321017891983893No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC4530.3134210635559799No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC4530.3134210635559799No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC4470.309269791191No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGAATACC4230.29266470173108056No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTCCTTCTTCTGCAGGTACC4090.2829783995461276No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3720.2573788866287517No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTGCGGGTACC3570.24700070571630206No Hit
TTACCGCGGCTGCTGGCACGTAGTTGGCCGGTGCTTCTTCTACGCATACC3530.2442331908063155No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC3460.23939003971383896No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTATTCCTCCGGTACC3170.21932555661643627No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT2880.19926107351903358No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGGTACT2840.19649355860904702No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2750.1902666500615772No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC2620.18127222660412082No Hit
TTACCGCGGCTGCTGGCACGGAGTTGGCCGTGGCTTATTCGGCAGGTACC2610.1805803478766242No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC2590.17919659042163089No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGACCTTATAAATAGTACCGT2590.17919659042163089No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTATCCAGCTACC2490.17227780314666444No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTCTGGTACC2480.1715859244191678No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTTGACGCGTACC2390.165359015871698No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATACAGGTACC2350.16259150096171143No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC2340.16189962223421478No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTTAGGTACC2170.15013768386677184No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC2160.1494458051392752No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCGTTAGGTACC2160.1494458051392752No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTCCTCACAAGGTACC2080.14391077531930202No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGTTACC2070.1432188965918054No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2040.14114326040931546No Hit
TTACCGCGGCTGCTGGCACGTAATTAGCCGGACCTTATTCGTATAGTACT2030.1404513816818188No Hit
TTACCGCGGCTGCTGGCACGAACTTAGCCCGTGCTTCCTCCAGGGATCGG2000.13837574549932888No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC1970.13630010931683895No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCCTTTAGCGGTACC1950.13491635186184567No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC1950.13491635186184567No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCCCATGGTACC1810.12523004967689264No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTACGGGTACC1790.12384629222189936No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTCTAGGTACC1750.12107877731191276No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATATAATACC1750.12107877731191276No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1750.12107877731191276No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGCCCCTTTCTGGTAAGATACC1700.11761938367442955No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1660.11485186876444298No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC1650.11415999003694634No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC1610.11139247512695974No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACC1520.10516556657948996No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCGTACAGTACC1510.10447368785199332No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC1460.10101429421451008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)