FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101122
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1991419.693044045806058No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1520215.033326081367063No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC49524.897055042424003No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC30903.0557148790569806No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC20732.0499990110955086No Hit
TTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC18951.8739740115899608No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC15461.5288463440200946No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC15291.512034967662823No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC13561.3409544906152964No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC11761.1629516821265402No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCAGGTACC10751.063072328474516No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTTACGATACC9730.9622040703308875No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTGCTGATTAGGTACC8520.8425466268467791No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTCCGGTACC7380.7298115148039002No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC6520.6447657285259389No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTTACCCATTACC5930.5864203635212911No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTACC5160.5102747176677677No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTGCTCCTACC4140.4094064595241391No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC3980.39358398765847197No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACC3920.38765056070884674No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC3780.37380589782638796No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTATCTAGGTACC3290.3253495777377821No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTTGCTTTCTGATTAGGTACC3130.30952710587211485No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCATCTGGTACC2820.27887106663238465No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTCTTCTGCTGGTACC2650.2620596902751132No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTCGGTACC2590.25612626332548805No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTCTTCTGCAGGTACT2480.2452483139178418No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCGGGTACC2450.2422816004430292No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCTCAGATACC2450.2422816004430292No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTCAGATACC2340.23140365103538302No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTATTCCGGTACC2340.23140365103538302No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTTCTCTGCAGGTACC2290.226459128577362No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTCTTCCGGTACC2250.22250351061094523No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTAACAAAGGTAC2070.20470322976206956No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGATACC2050.2027254207788612No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGATTAGGTACC1960.19382528035442337No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC1880.18591404442158976No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATAAGATACC1810.17899171298036037No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGACTAGGTACC1770.17503609501394354No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTGCTAAGGAGATACC1670.16514705009790154No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATGCTTATTCATACAGTACC1600.15822471865667215No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACC1520.15031348272383854No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGGTACC1500.14833567374063014No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATTAGGTAAC1460.14438005577421334No Hit
TTACCGCGGCTGCTGGCATGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1410.13943553331619232No Hit
CTACCGCGGCTGCTGGCACACACTGAGCCAGGATCAAACTCTTGTAGAAC1370.13547991534977552No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGCTTAGGTACC1350.13350210636656712No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCCTTAAGTACC1270.12559087043373351No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGGGCTTCTTCTCCGGGTACC1250.12361306145052511No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC1240.1226241569589209No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTCTCTGATTAGGTACC1230.12163525246731671No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGGGCTTTCTGATTAGGTACC1210.1196574434841083No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTACGCTACC1160.1147129210260873No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCGCTTTCTGGTAAGATACC1150.11372401653448311No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTAAGGTACC1140.11273511204287891No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGATAAGGTACC1140.11273511204287891No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC1140.11273511204287891No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTGGCTTTCTGATTAGGTACC1110.10976839856806629No Hit
TTACCGCCGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1090.1077905895848579No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCCTTCTGATTAGGTACC1080.10680168509325369No Hit
TTACCGCGGCTGCTGGCCCGTAGTTAGCCGTCCCTTTCTGGTAAGATACC1050.10383497161844109No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTAGCTTTCTGATTAGGTACC1050.10383497161844109No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGTGGCTTTCTGATTAGGTACC1040.10284606712683689No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGCCCTTTCTGGTAAGATACC1040.10284606712683689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)