FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002025673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209059
Sequences flagged as poor quality0
Sequence length300
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT8561940.95446739915526No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC2370011.336512659105804No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC111975.355904314093151No Hit
TTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC83694.00317613688002No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC63083.0173300360185404No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT44012.1051473507478753No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGGTACT24061.1508712851396017No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGCGCTTATTCTCCCGATACT22601.0810345404885702No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACT22451.073859532476478No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACC18150.8681759694631659No Hit
CTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC9370.44819883382203113No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCAGATACT6660.31857035573689724No Hit
TNACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTACGGTACC6330.30278533811029423No Hit
CTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC6010.287478654351164No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTCTTCTCCCGATACT5690.27217197059203385No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGCTACC4700.22481691771222478No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATAAT4650.22242524837486072No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTATTCTTCCGGTACC4460.21333690489287713No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCACGATACT4400.2104669016880402No Hit
CTACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC4160.1989868888686926No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGCTCCTTATTCTCACAGTACC4020.19229021472407312No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATCCTCCCGATACT3440.16454685041064962No Hit
CTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTAAC3420.163590182675704No Hit
TNACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCACAGTACC3310.158328510133503No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGAGCTTATTCTCCCGATACT3290.15737184239855734No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTACTCTCCCGATACT3270.1564151746636117No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGCTACT3240.15498017306119324No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGGGCTTATTCTCCCGATACT3040.14541349571173687No Hit
TACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATACTG2950.14110849090448152No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTATTCTTACGGTACC2890.13823848769964459No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCCTATTCTCCCGATACT2870.13728181996469896No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCCCCCGATACT2800.13393348289238924No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCTCCCGATCCT2750.13154181355502514No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGATCCTTATTCTCCCAGTACC2740.13106347968755233No Hit
TTACCGCGGCTGCTGGCATGGAGTTAGCCGGAGCTTATTCTCCCGATACT2650.12675847488029696No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGAGCTTATTCTCCCGATACT2540.12149680233809594No Hit
TTACCGCGGCTGCTGGCACGGGGTTAGCCGGAGCTTATTCTCCCGATACT2470.11814846526578622No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTACTCTTACGGTACC2440.11671346366336777No Hit
TTACCGCGGCTGCTGGCACGGAGTTAGCCGGAGCTTATTCGCCCGATACT2330.11145179112116675No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGGTGCTTCTTCTTACGGTACC2320.11097345725369394No Hit
TTACCGCGGCTGCTGGCACGGAGCTAGCCGGAGCTTATTCTCCCGATACT2300.1100167895187483No Hit
TTACCGCGGCTGCTGGCACGTAGTTAGCCGTCCCTTTCTGGTAAGATACC2200.1052334508440201No Hit
TNACCGCGGCTGCTGGCACGAAGTTAGCCGGTGCTTATTCTTTGGGTACC2200.1052334508440201No Hit
TTACCGCGGCTGCTGGCACAGAGTTAGCCGGTGCTTATTCTGTCGGTACC2110.10092844603676475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)