FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002025674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002025674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147355
Sequences flagged as poor quality0
Sequence length300
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATG5165535.05479963353805No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATG2177214.775202741678259No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATG1834012.446133487156866No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCCTAATACATG73775.006277357402192No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG50183.405381561535068No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATG35492.408469342743714No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCGTGCTTAACACATG20441.3871263275762613No Hit
AGAGTTTGATCCTGGCTCAGTGCCAGCAGCCGCGGTAAAGACCAAGTCTC13860.9405856604797936No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATG13820.9378711275491161No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCAGGCCTAACACATG8980.6094126429371246No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATG7750.525940755318788No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAATACATG7330.497438159546673No Hit
AGAGTTTGATCCTGGCTCAGTGTGTGCCAGCAGCCGCGGTAAAGACCAAG6930.4702928302398968No Hit
AGAGTTTGATCCTGGCTCAGTGTGCCAGCAGCCGCGGTAAAGACCAAGTC5960.40446540667096464No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTAACACATG5300.35967561331478404No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGTAGGCCTAACACATG5240.3556038139187676No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTATGCCTAACACATG4870.33049438430999967No Hit
AGAGTTTGATCCTGGCTCAGAATGAACGCTGGCGGTAGGCCTAACACATG4050.27484645923110856No Hit
AGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG3700.2510942960876794No Hit
AGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGTAGGCCTAACACATG2970.20155407010281293No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATG2770.18798140544942485No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACACG2660.18051643989006141No Hit
AGAGTTTGATCCTGGCTCGGAGTGAACGCTGGCGGTAGGCCTAACACATG2590.1757660072613756No Hit
AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAACACATG2580.1750873740287062No Hit
AGAGTTTGATCCTGGCTCAGAGTGGACGCTGGCGGTAGGCCTAACACATG2580.1750873740287062No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTGGGCCTAACACATG2520.17101557463268977No Hit
AGAGTTTGATCCTGGCTCAGAGTGAGCGCTGGCGGTAGGCCTAACACATG2360.1601574429099793No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGCATGCCTTACACATG2160.14658477825659122No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACGTG2120.1438702453259136No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCCGGCGGTAGGCCTAACACATG1940.13165484713786435No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACGCATG1790.12147534864782328No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCTTAACACATG1760.11943944894981506No Hit
AGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCAGGCTTAACACATG1740.11808218248447627No Hit
AGAGTTTGATCCTGGCTCAGGGTGAACGCTGGCGGTAGGCCTAACACATG1710.11604628278646806No Hit
AGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATG1540.1045095178310882No Hit
AGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAATACATG1480.10043771843507177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)